Techniques
Single Molecule Level
- expression (RT-PCR, Western)
- localization (Histology)
- activity (ELISA, radioimmunoassay)
Intact cell
- cell culture: brain astrocytes, lung microvascular endothelia, neonatal cardiomyocytes, etc.
- fluorometric recordings of Ca2+I transients in isolated adult cardiomyocytes (Indo-1AM)
- myocyte contraction (edge detection)
Intact Organ
- isolated perfused “working” heart
- postnatal (physiological) and pathological angiogenesis in the retina
- laser doppler perfusion imaging of blood flow
- vital microscopy analysis of permeability and blood flow in the microcirculation
- blood pressure measurements
- cardiac catheterization (see Lab of Prof. Kai Schuh)
- contractility of small blood vessels mounted in a myograph (see Lab of Prof. Andreas Friebe)
