Single Molecule Level

• expression (RT-PCR, Western)
• localization (Histology)
• activity (ELISA, radioimmunoassay)

Intact cell

• cell culture: brain astrocytes, lung microvascular endothelia, neonatal cardiomyocytes, etc.
• fluorometric recordings of Ca²⁺_I transients in isolated adult cardiomyocytes (Indo-1AM)
• myocyte contraction (edge detection)

Intact Organ

• isolated perfused “working” heart
• postnatal (physiological) and pathological angiogenesis in the retina
• laser doppler perfusion imaging of blood flow
• vital microscopy analysis of permeability and blood flow in the microcirculation
• blood pressure measurements
• cardiac catheterization (see Lab of Prof. Kai Schuh)
• contractility of small blood vessels mounted in a myograph (see Lab of Prof. Andreas Friebe)