Workgroup Kuhn


    Single Molecule Level

    • expression (RT-PCR, Western)
    • localization (Histology)
    • activity (ELISA, radioimmunoassay)

    Intact cell

    • cell culture: brain astrocytes, lung microvascular endothelia, neonatal cardiomyocytes, etc.
    • fluorometric recordings of Ca2+I transients in isolated adult cardiomyocytes (Indo-1AM)
    • myocyte contraction (edge detection)



    Intact Organ

    • isolated perfused “working” heart
    • postnatal (physiological) and pathological angiogenesis in the retina
    • laser doppler perfusion imaging of blood flow
    • vital microscopy analysis of permeability and blood flow in the microcirculation
    • blood pressure measurements
    • cardiac catheterization (see Lab of Prof. Kai Schuh)
    • contractility of small blood vessels mounted in a myograph (see Lab of Prof. Andreas Friebe)